Sperm penetration of immature and maturing oocytes does not affect phosphorylation of mitogen-activated protein kinase in pigs

dc.contributor	Quan, H M
dc.contributor	Meng, X Q
dc.contributor	Hou, Y
dc.contributor	Sun, Q Y
dc.date.accessioned	2012-01-30T12:42:52Z
dc.date.available	2012-01-30T12:42:52Z
dc.date.issued	2003
dc.identifier.citation	Rep. Fert. Dev. (2003) 15(7): 383-387
dc.identifier.issn	1031-3613
dc.identifier.uri	http://livestocklibrary.com.au/handle/1234/16492
dc.description.abstract	Pig oocytes cultured in vitro for 0, 25, 33 and 44�h were inseminated by frozen-thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase (MAPK) phosphorylation were evaluated. It was shown that: (1) oocytes at various maturational stages could be penetrated by sperm; (2) sperm penetration did not affect meiotic cell cycle progression; (3) sperm penetration of germinal vesicle (GV) oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I (pre-MI) and metaphase I (MI) oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes. The data suggest that sperm penetration before oocyte maturation does not affect MAPK phosphorylation and that the machinery inactivating MAPK upon fertilisation is not developed in maturing (pre-MI to MI) oocytes.
dc.publisher	CSIRO Publishing
dc.source.uri	http://www.publish.csiro.au/?act=view_file&file_id=RD03035.pdf
dc.subject	fertilisation
dc.subject	meiotic maturation
dc.title	Sperm penetration of immature and maturing oocytes does not affect phosphorylation of mitogen-activated protein kinase in pigs
dc.type	Research
dc.description.version	Journal article
dc.identifier.volume	15
dc.identifier.page	383-387
dc.identifier.issue	7